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one particular possible clarification for your elevated Aβ output from the Aph1 L30F/T164A mutant would be that the mutations enhance elaborate development. to check whether the Aph1 L30F/T164A mutants form a complex with other components, γ-secretase in yeast microsomes was immunoprecipitated using a PS1 antibody against the loop area (GIL3) (Figure 5).
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